Legionella pneumophila rapid detection
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Mas-Pons et al., in 2014, described a new technique to specifically label via click chemistry cultivable Legionella pneumophila membranes through assimilation of an azido analog of legionaminic acid precursor (Man2NAc4NAc6-N3). Legionaminic acid is an essential component of lipopolysaccharides (LPS) exclusively synthesized by Legionella.
The resulting viable Legionella pneumophila decorated with Man2NAc4NAc6-N3 at membrane surface can be detected via Cu(I)-catalyzed click chemistry or Cu-free click chemistry. Both offer the possibility to hook either an alkyne / cyclooctyne fluorescent dye or alkyne / cyclooctyne biotin followed by streptavidin enzyme conjugate for further detection by microscopy, colorimetry or luminescence. Alkyne / cyclooctyne biotin anchoring also allows subsequent isolation or enrichment process using streptavidin magnetic beads.
List of L. pneumophila strains tested:
Mas-Pons et al. Identification of living Legionella pneumophila using species-specific metabolic lipopolysaccharide labeling. Angew. Chem. Int. Ed., 2014,53(5):1275-8.
10 mg, 2,5 mg, 5 mg