flacon_Man2NAc4NAc6N3_click4tag

Man2NAc4NAc6-N3

150300

6-azido-2,4-diacetamido-2,4,6-trideoxy-D-mannopyranose
Legionella pneumophila rapid detection

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WeightPriceAdd to Cart
2,5 mg150
10 mg300

Product Description

Man2NAc4NAc6-N3 (6-azido-2,4-diacetamido-2,4,6-trideoxy-D-mannopyranose)

Mas-Pons et al., in 2014, described a new technique to specifically label via click chemistry cultivable Legionella pneumophila membranes through assimilation of an azido analog of legionaminic acid precursor (Man2NAc4NAc6-N3). Legionaminic acid is an essential component of lipopolysaccharides (LPS) exclusively synthesized by Legionella.

The resulting viable Legionella pneumophila decorated with Man2NAc4NAc6-Nat membrane surface can be detected via Cu(I)-catalyzed click chemistry or Cu-free click chemistry. Both offer the possibility to hook either an alkyne / cyclooctyne fluorescent dye or alkyne / cyclooctyne biotin followed by streptavidin enzyme conjugate for further detection by microscopy, colorimetry or luminescence. Alkyne / cyclooctyne biotin anchoring also allows subsequent isolation or enrichment process using streptavidin magnetic beads.

List of L. pneumophila strains tested:


Mas-Pons et al. Identification of living Legionella pneumophila using species-specific metabolic lipopolysaccharide labeling. Angew. Chem. Int. Ed.2014,53(5):1275-8.

Additional Information

Weight

10 mg, 2,5 mg, 5 mg