Gram negative bacteria labelling

Dumont et al.[1] described a new technique to label specifically membranes of culturable Gram negative bacteria, via click chemistry reaction, by assimilation of an azido analogue of Kdo (Kdo-N3), which is a lipopolysaccharide (LPS) essential component.

 

List of Gram negative bacteria tested:

Kdo-N3 Assimilation
16 hours
Alexa® alkyne Click

 


 

[1] Dumont A., Malleron A., Awwad M., Dukan S., Vauzeilles B., Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core,  Angew. Chem. Int. Ed.201251, 3143.