A real technological breakthrough, the approach developed by Click4Tag consists in specifically functionalizing the membrane of microorganisms of interest via the natural assimilation and then the integration of a « hook » onto this membrane. Afterwards, this anchorpoint allows, via a « click » reaction, to associate classical tags such as:
- A fluorescent probe to detect, enumerate and / or identify microorganisms of interest ,,
- Magnetic beads in order to concentrate / isolate specifically microorganisms of interest,
- According to the choice of probe, it is possible to obtain a specific detection. For example, Gram negative bacteria are « revealed » by the assimilation of a 3-deoxy-D-manno-octulosonic acid derivative .
Other probes are under development.
 Mas Pons J., Dumont A., Sautejeau G., Fugier E., Baron A., Dukan S., Vauzeilles B., Identification of Living Legionella pneumophila Using Species-Specific Metabolic Lipopolysaccharide Labeling, Angew. Chem. Int. Ed., 2014, 53, 1275.
 Dumont A., Malleron A., Awwad M., Dukan S., Vauzeilles B., Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core, Angew. Chem. Int. Ed., 2012, 51, 3143.